<<Training seminar for molecular detection of influenza virus>>

Date:            24^th -25^th September, 2014

Venue: Department of Medical Research, Upper Myanmar, Pyin Oo Lwin, Myanmar.

Course purpose: To learn basic skills and knowledge for detection of influenza virus by reverse transcription PCR and genetic sequencing.

List of tutors:

Reiko Saito, Professor, Division of International Health, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan

Hiroki Kondo, PhD candidate, Division of International Health, Graduate School of Medical and Dental Sciences, Niigata University

Go Hasegawa, Associate Professor, Division of Molecular Pathology, Graduate School of Medical and Dental Sciences, Niigata University

Tsutomu Tamura, Head of Division, Division of Virology, Niigata Prefectural Institute of Public Health and Environmental Sciences.

Host organizer:

Dr. Yi Yi Myint, Director General, Upper Myanmar, Pyin Oo Lwin, Myanmar.

Day 1: 24^th September (Wed), 9:00- 16:00

9:00-12:00: morning session

(1) Opening remarks

Yi Yi Myint

Director General, Department of Medical Research, Upper Myanmar

(2)  Introduction of molecular detection methods

Reiko Saito

Division of International Health, Niigata University

(3) 

Go Hasegawa

Division of Molecular Pathology

(4) Training of RNA extraction and complementary DNA synthesis (incl. talk and demonstration)

Hiroki Kondo, Reiko Saito, Tsutomu Tamura, Go Hasegawa

Niigata University and Niigata Prefectural Institute of Public Health and Environmental Sciences

12:00-13:30  Lunch Break

13:30-16:00  Afternoon session

(4-1) Course A: Training of PCR for influenza A detection (incl. talk and demonstration)

Hiroki Kondo and Reiko Saito

(4-2) Course B: Training of Genetic Sequencing (incl. talk and demonstration)

Tsutomu Tamura and Go Hasegawa

Day 2: 25^th September (Thu), 9:00-12:00

9:00- 12:00 Morning session

(5-1) Course A: Training of PCR for influenza B detection (incl. talk and demonstration)

Hiroki Kondo and Reiko Saito

(5-2) Course B: Training of Genetic Sequencing (incl. talk and demonstration)-continued

Tsutomu Tamura and Go Hasegawa

(6) Closing remarks

Yi Yi Myint

Director General, Department of Medical Research, Upper Myanmar

This seminar is funded by Japan Society for the Promotion of Science (JSPS) Core to Core Program, B. Asia-Africa Science Platforms, program entitled “Analyzing circulation pattern of influenza virus in tropical and subtropical Asia to contribute to global prevention and control of influenza”