{"id":5919,"date":"2023-05-29T15:24:46","date_gmt":"2023-05-29T06:24:46","guid":{"rendered":"http:\/\/www.med.niigata-u.ac.jp\/pub\/?p=5919"},"modified":"2023-05-29T15:24:46","modified_gmt":"2023-05-29T06:24:46","slug":"2023%e5%b9%b45%e6%9c%8823%e6%97%a5%e3%80%80%e6%8a%97%e3%82%a4%e3%83%b3%e3%83%95%e3%83%ab%e3%82%a8%e3%83%b3%e3%82%b6%e5%89%a4%e6%84%9f%e5%8f%97%e6%80%a7%e4%bd%8e%e4%b8%8b%e6%a0%aa%e8%aa%bf%e6%9f%bb","status":"publish","type":"post","link":"http:\/\/www.med.niigata-u.ac.jp\/pub\/2023\/05\/29\/2023%e5%b9%b45%e6%9c%8823%e6%97%a5%e3%80%80%e6%8a%97%e3%82%a4%e3%83%b3%e3%83%95%e3%83%ab%e3%82%a8%e3%83%b3%e3%82%b6%e5%89%a4%e6%84%9f%e5%8f%97%e6%80%a7%e4%bd%8e%e4%b8%8b%e6%a0%aa%e8%aa%bf%e6%9f%bb\/","title":{"rendered":"2023\u5e745\u670823\u65e5\u3000\u6297\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u5264\u611f\u53d7\u6027\u4f4e\u4e0b\u682a\u8abf\u67fb\u306e\u7d50\u679c"},"content":{"rendered":"

\u7d50\u679c\u306b\u3064\u3044\u3066<\/p>\n

2022\/23\u30b7\u30fc\u30ba\u30f3\u4e2d\u30012023\u5e745\u670823\u65e5\u6642\u70b9\u3067\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u69d8\u75be\u60a3\u306e\u60a3\u8005\u304b\u3089\u63a1\u53d6\u3055\u308c\u305f\u30b5\u30f3\u30d7\u30eb\u306f\u4ee5\u4e0b\u306e\u3068\u304a\u308a\u3067\u3059\u3002<\/p>\n

\u5168\u56fd\u304b\u3089\u3001\u521d\u8a3a\u6642\u3067 199\u4ef6\u3001\u518d\u8a3a\u6642\u3067 82 \u4ef6\u3001\u30d0\u30ed\u30ad\u30b5\u30d3\u30eb\u6cbb\u7642\u5f8c\u306b 51 \u4ef6\u304c\u53ce\u96c6\u3055\u308c\u307e\u3057\u305f (\u8868 1)\u3002<\/p>\n

\"\"<\/a><\/p>\n

\u30b5\u30a4\u30af\u30ea\u30f3\u30b0\u30d7\u30ed\u30fc\u30d6\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0 PCR \u306b\u3088\u308b PA\/I38T \u5909\u7570\u306e\u691c\u51fa\u7d50\u679c\u3092\u4ee5\u4e0b\u306b\u793a\u3057\u307e\u3059 (\u8868 2)\u3002<\/p>\n

\"\"<\/a><\/p>\n

PA\/I38T \u5909\u7570\u304c\u691c\u51fa\u3055\u308c\u305f\u60a3\u8005\u306e\u5272\u5408\u306f\u6b21\u306e\u3068\u304a\u308a\u3067\u3059\u3002<\/p>\n

A(H1N1)pdm09 \u3067\u306f\u30011 \u4ef6\u4e2d\u3001\u30d0\u30ed\u30ad\u30b5\u30d3\u30eb\u6cbb\u7642\u524d\u304a\u3088\u3073\u6cbb\u7642\u5f8c\u306e\u30b5\u30f3\u30d7\u30eb\u3067 PA\/I38T \u5909\u7570\u306f\u691c\u51fa\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f (0.0%)\u3002 A(H3N2) \u3067\u306f\u3001\u30d0\u30ed\u30ad\u30b5\u30d3\u30eb\u6cbb\u7642\u524d\u30b5\u30f3\u30d7\u30eb\u306e\u3046\u3061 178 \u4ef6\u4e2d 1 \u4ef6\u304b\u3089PA\/I38T \u5909\u7570(0.5%) \u304c\u691c\u51fa\u3055\u308c\u307e\u3057\u305f\u3002 \u6cbb\u7642\u5f8c\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u306f\u300150 \u4ef6\u4e2d 3 \u4ef6\u3067PA\/I38T \u5909\u7570 (6.0%) \u304c\u691c\u51fa\u3055\u308c\u307e\u3057\u305f\u3002 \u518d\u8a3a\u6642 RT-PCR \u967d\u6027\u8005\u6570\u306b\u57fa\u3065\u3044\u3066\u983b\u5ea6\u3092\u8a08\u7b97\u3059\u308b\u3068\u300115.0% \u3068\u306a\u308a\u307e\u3057\u305f (\u518d\u8a3a\u6642\u306e 20 \u4ef6\u306e RT-PCR \u967d\u6027\u30b5\u30f3\u30d7\u30eb\u306e\u3046\u3061 3 \u4ef6\u306e PA\/I38T \u5909\u7570)\u3002<\/p>\n

\u4e9c\u578b\u304c\u4e0d\u660e\u306a\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6 A \u30b5\u30f3\u30d7\u30eb\u3067\u306f\u3001\u6cbb\u7642\u524d\u306e\u30b5\u30f3\u30d7\u30eb\u306f 9 \u4ef6 \u3059\u3079\u3066\u3067PA\/I38T \u5909\u7570(0%) \u304c\u691c\u51fa\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/p>\n

\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6B\u578b\u306f\u691c\u51fa\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/p>\n

 <\/p>\n

\u3010PA\u907a\u4f1d\u5b50\u89e3\u6790\u3011<\/p>\n

A(H3N2)\u3067\u306f\u300139\u4ef6\u306e\u30d0\u30ed\u30ad\u30b5\u30d3\u30eb\u6295\u4e0e\u81e8\u5e8a\u30b5\u30f3\u30d7\u30eb\u306e\u3046\u3061\u30011\u4ef6\u3067PA\/I38M\u5909\u7570\u30012\u4ef6\u3067I38T\u5909\u7570(7.7%)\u304c\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b7\u30f3\u30b0(NGS)\u306b\u3088\u3063\u3066\u691c\u51fa\u3055\u308c\u307e\u3057\u305f(\u88683)\u3002 \u5206\u6bcd\u304c\u30d0\u30ed\u30ad\u30b5\u30d3\u30eb\u6cbb\u7642\u7fa4\u3067NGS\u304c\u6210\u529f\u3057\u305f8\u4ef6\u306b\u57fa\u3065\u304f\u5834\u5408\u3001PA\u5909\u7570\u306e\u983b\u5ea6\u306f37.5%\u3067\u3057\u305f\u3002<\/p>\n

\"\"<\/a><\/p>\n

\u3010NA\u907a\u4f1d\u5b50\u89e3\u6790\u3011<\/p>\n

NAI \u306b\u5bfe\u3059\u308b\u611f\u53d7\u6027\u306e\u4f4e\u4e0b\u3092\u3082\u305f\u3089\u3059 NA \u907a\u4f1d\u5b50\u306e\u7f6e\u63db\u306f\u3001\u30aa\u30bb\u30eb\u30bf\u30df\u30d3\u30eb\u6cbb\u7642\u524d\u30b5\u30f3\u30d7\u30eb (0\/118) \u307e\u305f\u306f\u6cbb\u7642\u5f8c\u306e\u30b5\u30f3\u30d7\u30eb (0\/29) \u306e\u3044\u305a\u308c\u306b\u304a\u3044\u3066\u3082\u3001NGS\u306b\u3088\u3063\u3066\u691c\u51fa\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f (\u8868 4)\u3002<\/p>\n

\"\"<\/a><\/p>\n

Summary of Results.
\nDuring 2022\/23 season, samples collected from influenza-like-illness patients as of May 23, 2023 were as below:<\/p>\n

From main island of Japan and Okinawa 199 samples were collected at the first visit, 82 samples at the second visit, 51 samples after baloxavir treatment (Table 1).<\/p>\n

 <\/p>\n

The results of the detection of PA\/I38T substation by cycling probe real-time PCR are shown below (Table 2).<\/p>\n

The percentage of patients with PA\/I38T substitution was:
\nIn A(H1N1)pdm09, no PA\/I38T variant in pre- and post-treatment samples was detected out of 1 sample (0.0%). In A(H3N2), among pre-treatment samples 1 PA\/I38T variant (0.5%) was detected out of 178 samples. Among post baloxavir-treatment samples, 3 PA\/I38T variants (6.0%) were detected out of 50 samples. If the frequency is calculated based on RT-PCR-positives at the second visit, it became 15.0% (3 PA\/I38T variants out of 20 RT-PCR-positive samples at the second visit).<\/p>\n

In unsubtyped influenza A, pre-treatment samples were 0 positive (0%) PA\/I38T variant out of 9 specimens.<\/p>\n

Influenza B was not detected.<\/p>\n

 <\/p>\n

[PA gene analysis]<\/p>\n

In A(H3N2), one PA\/I38M, and two I38T substitutions (7.7%) out of 39 paired baloxavir-treated clinical sample was detected by next-generation sequencing (Table 3). If the denominator is based on 8 samples with successful NGS in the baloxavir-treated group, the frequency of PA variant was 37.5%.<\/p>\n

 <\/p>\n

[NA gene analysis]<\/p>\n

No substitution in NA gene which confer reduced sensitivity to NAIs was found by next-generation sequencing either in pre-treatment samples (0\/118) or post-oseltamivir-treated samples (0\/29) (Table 4).<\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d50\u679c\u306b\u3064\u3044\u3066 2022\/23\u30b7\u30fc\u30ba\u30f3\u4e2d\u30012023\u5e745\u670823\u65e5\u6642\u70b9\u3067\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u69d8\u75be\u60a3\u306e\u60a3\u8005\u304b\u3089\u63a1\u53d6\u3055\u308c\u305f\u30b5\u30f3\u30d7\u30eb\u306f\u4ee5\u4e0b\u306e\u3068\u304a\u308a\u3067\u3059\u3002 \u5168\u56fd\u304b\u3089\u3001\u521d\u8a3a\u6642\u3067 199\u4ef6\u3001\u518d\u8a3a\u6642\u3067 82 \u4ef6\u3001\u30d0\u30ed\u30ad\u30b5\u30d3\u30eb\u6cbb\u7642\u5f8c\u306b 51 \u4ef6\u304c\u53ce 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