{"id":6141,"date":"2024-02-26T17:52:29","date_gmt":"2024-02-26T08:52:29","guid":{"rendered":"http:\/\/www.med.niigata-u.ac.jp\/pub\/?p=6141"},"modified":"2024-02-27T18:27:41","modified_gmt":"2024-02-27T09:27:41","slug":"2024%e5%b9%b42%e6%9c%8816%e6%97%a5%e3%80%80%e6%8a%97%e3%82%a4%e3%83%b3%e3%83%95%e3%83%ab%e3%82%a8%e3%83%b3%e3%82%b6%e5%89%a4%e6%84%9f%e5%8f%97%e6%80%a7%e4%bd%8e%e4%b8%8b%e6%a0%aa%e8%aa%bf%e6%9f%bb","status":"publish","type":"post","link":"http:\/\/www.med.niigata-u.ac.jp\/pub\/2024\/02\/26\/2024%e5%b9%b42%e6%9c%8816%e6%97%a5%e3%80%80%e6%8a%97%e3%82%a4%e3%83%b3%e3%83%95%e3%83%ab%e3%82%a8%e3%83%b3%e3%82%b6%e5%89%a4%e6%84%9f%e5%8f%97%e6%80%a7%e4%bd%8e%e4%b8%8b%e6%a0%aa%e8%aa%bf%e6%9f%bb\/","title":{"rendered":"2024\u5e742\u670816\u65e5\u3000\u6297\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u5264\u611f\u53d7\u6027\u4f4e\u4e0b\u682a\u8abf\u67fb\u306e\u7d50\u679c"},"content":{"rendered":"

2024\u5e742\u670816\u65e5\u6642\u70b9<\/p>\n

2023\u5e7410\u6708\u304b\u30892024\u5e742\u6708\u306b\u304b\u3051\u3066\u3001\u5408\u8a0894\u4ef6\u306e\u521d\u8a3a\u6642\u691c\u4f53\u304c\u63a1\u53d6\u3055\u308c\u307e\u3057\u305f\u3002\u518d\u8a3a\u6642\u691c\u4f53\u306f30\u4ef6\u63a1\u53d6\u3055\u308c\u3001\u3046\u306118\u4ef6\u306f\u30d0\u30ed\u30ad\u30b5\u30d3\u30eb\u6295\u4e0e\u5f8c\u306b\u63a1\u53d6\u3055\u308c\u307e\u3057\u305f\uff08\u88681\uff09\u3002\"\"<\/a><\/p>\n

 <\/p>\n

2023\u5e7410\u6708\u4ee5\u964d\u3001\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6A(H1N1)pdm09\u309239\u4ef6\u3001A(H3N2)\u309235\u4ef6\u3001\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6A\u578b\uff08\u4e9c\u578b\u304c\u7279\u5b9a\u3067\u304d\u306a\u3044\u3001\u53c8\u306fA(H1N1)pdm09\u3068A(H3N2)\u306e\u6df7\u5408\u682a\uff09\u30928\u4ef6\u3001B\/\u30d3\u30af\u30c8\u30ea\u30a2\u30921\u4ef6\u691c\u51fa\u3057\u307e\u3057\u305f\uff08\u88681\uff0c\u56f31\uff09\u3002\"\"<\/a><\/p>\n

\u4eca\u30b7\u30fc\u30ba\u30f3\u306fA(H1N1)pdm09\u3068A(H3N2)\u306e\u540c\u6642\u6d41\u884c\u3067\u3042\u308a\u3001\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u691c\u51fa\u6570\u306f11\u6708\u304c\u6700\u3082\u591a\u304f\u300112\u6708\u4ee5\u964d\u306f\u5f90\u3005\u306b\u6e1b\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n

 <\/p>\n

\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30a6\u30a4\u30eb\u30b9\u306ePA\u86cb\u767d38\u4f4d\u304c\u30a4\u30bd\u30ed\u30a4\u30b7\u30f3\u304b\u3089\u30b9\u30ec\u30aa\u30cb\u30f3\u306b\u5909\u7570\u3057\u305f\u30a6\u30a4\u30eb\u30b9(PA\/I38T\u5909\u7570\u682a)\u306f\u3001\u30d0\u30ed\u30ad\u30b5\u30d3\u30eb\u306b\u5bfe\u3057\u3066\u611f\u53d7\u6027\u4f4e\u4e0b\u3092\u793a\u3059\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u3053\u306ePA\/I38T\u5909\u7570\u682a\u3092\u7279\u7570\u7684\u306b\u691c\u51fa\u3059\u308b\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u6cd5\uff08\u30b5\u30a4\u30af\u30ea\u30f3\u30d6\u30d7\u30ed\u30fc\u30d6\u6cd5\uff09\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002\u6297\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u85ac\u6295\u4e0e\u524d\u306eA(H1N1)pdm09\u306e39\u682a\u3001A(H3N2)\u306e35\u682a\u3001B\u578b1\u682a\u306e\u5168\u3066\u306b\u3001PA\/I38T\u5909\u7570\u682a\u306f\u691c\u51fa\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\uff08\u88682\uff09\u3002\"\"<\/a><\/p>\n

 <\/p>\n

\u30d0\u30ed\u30ad\u30b5\u30d3\u30eb\u6295\u4e0e\u5f8c\u306b\u63a1\u53d6\u3055\u308c\u305f\u691c\u4f53\uff08\u518d\u8a3a\u6642\u691c\u4f53\uff09\u306f18\u691c\u4f53\u3067\u3057\u305f\uff08\u88682\uff09\u300212\u4ef6\u304cA(H1N1)pdm09\u3067\u3001\u3046\u30614\u4ef6\u304cPCR\u967d\u6027\u3067\u3057\u305f\u304c\u3001PA\/I38T\u5909\u7570\u306f\u691c\u51fa\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002A(H3N2)\u306f6\u4ef6\u3067\u3057\u305f\u304c\u3001PCR\u306f\u5168\u3066\u9670\u6027\u3067\u3057\u305f\u3002\u3053\u306e\u305f\u3081\u3001PA\/I38T\u5909\u7570\u682a\u306f\u518d\u8a3a\u6642\u306b\u3082\u691c\u51fa\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/p>\n

 <\/p>\n

[PA\u907a\u4f1d\u5b50\u89e3\u6790\uff3d<\/p>\n

\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b7\u30f3\u30b0\uff08NGS\uff09\u3092\u5b9f\u65bd\u3057\u305f\u521d\u8a3a\u6642\u691c\u4f537\u4ef6\u306f\u3001A(H1N1)pdm09\u304c5\u4ef6\u3001A(H3N2)\u304c1\u4ef6\u3001B\u578b\u304c1\u4ef6\u3067\u3057\u305f\u304c\u3001\u30d0\u30ed\u30ad\u30b5\u30d3\u30eb\u306b\u5bfe\u3059\u308b\u611f\u53d7\u6027\u4f4e\u4e0b\u3092\u793a\u3059\u3068\u5831\u544a\u3055\u308c\u305f\u5909\u7570\u306fPA\u86cb\u767d\u306b\u691c\u51fa\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\uff08\u88683\uff09\u3002\"\"<\/a><\/p>\n

 <\/p>\n

[NA\u907a\u4f1d\u5b50\u89e3\u6790\uff3d<\/p>\n

NGS\u306e\u7d50\u679c\u3001A(H1N1)pdm09\u306e5\u4ef6\u306b\u306f\u3001NA\u963b\u5bb3\u5264\u306b\u5bfe\u3059\u308b\u611f\u53d7\u6027\u3092\u4f4e\u4e0b\u3055\u305b\u308b\u5909\u7570\u306fNA\u907a\u4f1d\u5b50\u306b\u691c\u51fa\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\uff08\u88684\uff09\u3002\"\"<\/a><\/p>\n

 <\/p>\n

 <\/p>\n

Summary of Results.
\nAs of February 16, 2024<\/p>\n

From October 2023 to February 2024, a total of 94 initial visit specimens were collected. There were 30 specimens collected at follow-up visits (second visits), of which 18 were collected after administration of baloxavir (Table 1).<\/p>\n

Since October 2023, 39 cases of Influenza A(H1N1)pdm09, 35 cases of A(H3N2), 8 cases of Influenza A t (subtype unidentified or mixed strains of A(H1N1)pdm09 and A(H3N2)), and 1 case of B\/Victoria were detected (Table 1 and Figure 1). This season saw a simultaneous circulation of A(H1N1)pdm09 and A(H3N2), with the highest number of influenza detections in November, gradually decreasing from December onwards.<\/p>\n

A virus with a substitution at position 38 of the PA protein, from isoleucine to threonine (PA\/I38T mutation), is reported to confer reduced sensitivity to baloxavir. We implemented a real-time PCR method (cycling probe method) specifically designed to detect this PA\/I38T mutation. None of the 39 of A(H1N1)pdm09, 35 of A(H3N2), and 1 of influenza B collected before administration of antiviral influenza drugs showed the presence of the PA\/I38T mutation (Table 2).<\/p>\n

There were 18 specimens collected after administration of baloxavir (follow-up visit specimens) (Table 2). Of these, 12 were A(H1N1)pdm09, of which 4 were PCR positive, but the PA\/I38T substitution was not detected. There were 6 cases of A(H3N2), all PCR negative at the follow-up specimens. Therefore, the PA\/I38T variant strain was not detected in the follow-up visit samples.<\/p>\n

[PA Gene Analysis]<\/p>\n

Seven initial visit specimens were subjected to next-generation sequencing (NGS), with 5 cases of A(H1N1)pdm09, 1 case of A(H3N2), and 1 case of type B. However, substitution reported to confer reduced sensitivity to baloxavir were not detected in the PA protein (Table 3).<\/p>\n

[NA Gene Analysis]<\/p>\n

NGS results showed no substitution in the NA gene affecting sensitivity to NA inhibitors in the 5 cases of A(H1N1)pdm09 (Table 4).<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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