© May 2024 Division of Microscopic Anatomy, Graduate School of Medical and Dental Sciences, Niigata University
Microscopic Anatomy, Niigata University
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牛木客員教授研究室の業績


2021年


1. Ushiki T, Ishizaki K, Mizutani Y, Nakajima M, Iwata F: Scanning ion conductance microscopy of isolated metaphase chromosomes in a liquid environment. Chromosome Res.2021 Mar;29(1):95-106. doi: 10.1007/s10577-021-09659-0. Epub 2021 Mar 11. PMID: 33694044
2. Iwata F, Shirasawa T, Mizutani Y, Ushiki T.: Scanning ion-conductance microscopy with a double-barreled nanopipette for topographic imaging of charged chromosomes. Microscopy (Oxf). 2021 Oct 5;70(5):423-435. doi: 10.1093/jmicro/dfab009.



2020年


3. Mizutani Y, Omagari D, Hayatsu M, Nameta M, Komiyama K, Mikami Y, Ushiki T.: SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role. Cell Adh Migr. 2020 Dec;14(1):195-203. doi: 10.1080/19336918.2020.1829264. PMID: 33016205
4. Matsugishi A, Aoki-Nonaka Y, Yokoji-Takeuchi M, Yamada-Hara M, Mikami Y, Hayatsu M, Terao Y, Domon H, Taniguchi M, Takahashi N, Yamazaki K, Tabeta K.: Rice peptide with amino acid substitution inhibits biofilm formation by Porphyromonas gingivalis and Fusobacterium nucleatum. Arch Oral Biol. 2021 Jan;121:104956. doi: 10.1016/j.archoralbio.2020.104956. Epub 2020 Oct 30. PMID: 33157493
5. Omagari D, Hayatsu M, Yamamoto K, Kobayashi M, Tsukano N, Nameta M, Mikami Y.: Gap junction with MLO-A5 osteoblast-like cell line induces ALP and BSP transcription of 3T3-L1 pre-adipocyte like cell line via Hspb1 while retaining adipogenic differentiation ability. Bone. 2020 Dec;141:115596. doi: 10.1016/j.bone.2020.115596. Epub 2020 Aug 16. PMID: 32814124
6. Nagashima H, Koga D, Kusumi S, Mukaigasa K, Yaginuma H, Ushiki T, Sato N.: Novel concept for the epaxial/hypaxial boundary based on neuronal development. J Anat. 2020 Sep;237(3):427-438. doi: 10.1111/joa.13219. Epub 2020 Aug 12. PMID: 32786168
7. Yoshioka N, Kabata Y, Kuriyama M, Bizen N, Zhou L, Tran DM, Yano M, Yoshiki A, Ushiki T, Sproule TJ, Abe R, Takebayashi H.: Diverse dystonin gene mutations cause distinct patterns of Dst isoform deficiency and phenotypic heterogeneity in Dystonia musculorum mice. Dis Model Mech. 2020 May 21;13(5):dmm041608. doi: 10.1242/dmm.041608. PMID: 32482619
8. Arao Y, Kawai H, Kamimura K, Kobayashi T, Nakano O, Hayatsu M, Ushiki T, Terai S.: Effect of methionine/choline-deficient diet and high-fat diet-induced steatohepatitis on mitochondrial homeostasis in mice. Biochem Biophys Res Commun. 2020 Jun 25;527(2):365-371. doi: 10.1016/j.bbrc.2020.03.180. Epub 2020 Apr 10. PMID: 32284169
9. Kawai T, Kabata Y, Shinkuma S, Oginezawa M, Hayashi R, Hayatsu M, Abe R.: Intracytoplasmic abnormality of corneocytes in circumscribed palmar or plantar hypokeratosis: ultrastructural observations. J Eur Acad Dermatol Venereol. 2020 Nov;34(11):e709-e711. doi: 10.1111/jdv.16518. Epub 2020 Jun 1. PMID: 32314449



2019年


10. Morita K, Azumi R, Sato M, Mizutani Y, Hayatsu M, Terai S, Ushiki T.: Dynamic changes in basal lamina fenestrations in rat intestinal villous epithelium under high-fat diet condition. Biomed Res. 2019;40(2):57-66. doi: 10.2220/biomedres.40.57. PMID: 30982801
11. Seino Y, Nakatomi M, Ida-Yonemochi H, Koga D, Ushiki T, Ohshima H.: Three-dimensional configuration of apical epithelial compartments including stem cell niches in guinea pig cheek teeth. J Oral Biosci. 2019 Mar;61(1):55-63. doi: 10.1016/j.job.2019.01.002. Epub 2019 Jan 21. PMID: 30929803
12. Kabata Y, Orime M, Abe R, Ushiki T.: The morphology, size and density of the touch dome in human hairy skin by scanning electron microscopy. Microscopy (Oxf). 2019 Jun 1;68(3):207-215. doi: 10.1093/jmicro/dfz001. PMID: 30860586



2018年


13. Mikami Y, Omagari D, Mizutani Y, Hayatsu M, Ushiki T, Tsuda H.: Dual effect of polyphosphate on mineralization of rat osteoblast ROS17/2.8 cells in a dose-dependent manner. J Pharmacol Sci. 2018 Nov;138(3):209-213. doi: 10.1016/j.jphs.2018.10.002. Epub 2018 Oct 13. PMID: 30389276
14. Yamada M, Takahashi N, Matsuda Y, Sato K, Yokoji M, Sulijaya B, Maekawa T, Ushiki T, Mikami Y, Hayatsu M, Mizutani Y, Kishino S, Ogawa J, Arita M, Tabeta K, Maeda T, Yamazaki K.: A bacterial metabolite ameliorates periodontal pathogen-induced gingival epithelial barrier disruption via GPR40 signaling. Sci Rep. 2018 Jun 13;8(1):9008. doi: 10.1038/s41598-018-27408-y. PMID: 29899364
15. Azumi R, Morita K, Mizutani Y, Hayatsu M, Terai S, Ushiki T.: Dynamics of basal lamina fenestrations in the rat intestinal villous epithelium in response to dietary conditions. Biomed Res. 2018;39(2):65-74. doi: 10.2220/biomedres.39.65. PMID: 29669985



2017年


16. Koga D, Kusumi S, Ushiki T, Watanabe T.: Integrative method for three-dimensional imaging of the entire Golgi apparatus by combining thiamine pyrophosphatase cytochemistry and array tomography using backscattered electron-mode scanning electron microscopy. Biomed Res. 2017;38(5):285-296. doi: 10.2220/biomedres.38.285. PMID: 29070778
17. Nakajima M, Mizutani Y, Iwata F, Ushiki T.: Scanning ion conductance microscopy for visualizing the three-dimensional surface topography of cells and tissues. Semin Cell Dev Biol. 2018 Jan;73:125-131. doi: 10.1016/j.semcdb.2017.09.024. Epub 2017 Oct 5. PMID: 28939037
18. Murofushi T, Tsuda H, Mikami Y, Yamaguchi Y, Suzuki N.: CAY10591, a SIRT1 activator, suppresses cell growth, invasion, and migration in gingival epithelial carcinoma cells. J Oral Sci. 2017;59(3):415-423. doi: 10.2334/josnusd.16-0696. PMID: 28904318
19. Shodo R, Hayatsu M, Koga D, Horii A, Ushiki T.: Three-dimensional reconstruction of root cells and interdental cells in the rat inner ear by serial section scanning electron microscopy. Biomed Res. 2017;38(4):239-248. doi: 10.2220/biomedres.38.239. PMID: 28794401
20. Takamura T, Suguro H, Mikami Y, Iwase T, Komiyama Y, Kuyama K, Komiyama K, Oki H.: Comparison of gene expression profiles of gingival carcinoma Ca9-22 cells and colorectal adenocarcinoma HT-29 cells to identify potentially important mediators of SLPI-induced cell migration. J Oral Sci. 2017;59(2):279-287. doi: 10.2334/josnusd.16-0534. PMID: 28637988
21. Koga D, Ushiki T, Watanabe T.: Novel scanning electron microscopy methods for analyzing the 3D structure of the Golgi apparatus. Anat Sci Int. 2017 Jan;92(1):37-49. doi: 10.1007/s12565-016-0380-8. Epub 2016 Oct 26. PMID: 27785745
22. Evans M, Murofushi T, Tsuda H, Mikami Y, Zhao N, Ochiai K, Kurita-Ochiai T, Yamamoto M, Otsuka K, Suzuki N.: Combined effects of starvation and butyrate on autophagy-dependent gingival epithelial cell death. J Periodontal Res. 2017 Jun;52(3):522-531. doi: 10.1111/jre.12418. Epub 2016 Sep 13. PMID: 27624546



2016年


23. Mikami Y, Fukushima A, Komiyama Y, Iwase T, Tsuda H, Higuchi Y, Hayakawa S, Kuyama K, Komiyama K.: Human uterus myoma and gene expression profiling: A novel in vitro model for studying secretory leukocyte protease inhibitor-mediated tumor invasion. Cancer Lett. 2016 Aug 28;379(1):84-93. doi: 10.1016/j.canlet.2016.05.028. Epub 2016 May 26. PMID: 27238568
24. Kariya T, Ueta H, Xu XD, Koga D, Ezaki T, Yu E, Kusumi S, Kitazawa Y, Sawanobori Y, Ushiki T, Issekutz T, Matsuno K.: Direct evidence for activated CD8+ T cell transmigration across portal vein endothelial cells in liver graft rejection. J Gastroenterol. 2016 Oct;51(10):985-98. doi: 10.1007/s00535-016-1169-1. Epub 2016 Feb 18. PMID: 26891909
25. Koga D, Bochimoto H, Watanabe T, Ushiki T.: Backscattered electron image of osmium-impregnated/macerated tissues as a novel technique for identifying the cis-face of the Golgi apparatus by high-resolution scanning electron microscopy. J Microsc. 2016 Jul;263(1):87-96. doi: 10.1111/jmi.12379. Epub 2016 Jan 25. PMID: 26807791



2015年


26. Koga D, Kusumi S, Ushiki T.: Three-dimensional shape of the Golgi apparatus in different cell types: serial section scanning electron microscopy of the osmium-impregnated Golgi apparatus. Microscopy (Oxf). 2016 Apr;65(2):145-57. doi: 10.1093/jmicro/dfv360. Epub 2015 Nov 24. PMID: 26609075
27. Koga D, Kusumi S, Bochimoto H, Watanabe T, Ushiki T.: Correlative Light and Scanning Electron Microscopy for Observing the Three-Dimensional Ultrastructure of Membranous Cell Organelles in Relation to Their Molecular Components. J Histochem Cytochem. 2015 Dec;63(12):968-79. doi: 10.1369/0022155415609099. Epub 2015 Sep 15. PMID: 26374827
28. Koga D, Kusumi S, Shodo R, Dan Y, Ushiki T.: High-resolution imaging by scanning electron microscopy of semithin sections in correlation with light microscopy. Microscopy (Oxf). 2015 Dec;64(6):387-94. doi: 10.1093/jmicro/dfv042. Epub 2015 Jul 22. PMID: 26206941
29. Kusumi S, Koga D, Kanda T, Ushiki T.: Three-dimensional reconstruction of serial sections for analysis of the microvasculature of the white pulp and the marginal zone in the human spleen. Biomed Res. 2015;36(3):195-203. doi: 10.2220/biomedres.36.195. PMID: 26106049



2014年


30. Watanabe T, Bochimoto H, Koga D, Hosaka M, Ushiki T.: Functional implications of the Golgi and microtubular network in gonadotropes. Mol Cell Endocrinol. 2014 Mar 25;385(1-2):88-96. doi: 10.1016/j.mce.2013.10.003. Epub 2013 Oct 10. PMID: 24121198



2013年


31. Iwata F, Y Ohashi, I Ishisaki, LM Picco, T Ushiki: Development of nanomanipulator using a high-speedatomic force microscope coupled with a haptic device. Ultramicroscopy 2013 Oct;133:88-94. doi: 10.1016/j.ultramic.2013.06.014.Epub 2013 Jul 12.[PubMed - in process]
32. Orime M, T Ushiki, D Koga, M Ito: Three-Dimensional Morphology of Touch Domes in Human Hairy Skin by Correlative Light and Scanning Electron Microscopy. J Invest Dermatol. 133: 2018-2011 (2013), Feb 4. doi:10.1038/jid.2013.60. [Epub ahead of print]
33. Iwata F, Y Mizuguchi, H Ko, T Ushiki: A compact nano manipulator based on an atomic force microscope coupling with a scanning electron microscope or an inverted optical microscope. J Micro-Bio Robot.DOI 10.1007/s12213-013-0063



2012年


34. Koga D, M Nakajima, T Ushiki: A useful method for observing intracellular structures of free and cultured cells by scanning electron microscopy. J Electron Microsc (Tokyo). 61(2):105-111(2012).[PubMed]
35. Ushiki T, M Nakajima, M Choi, SJ Cho, F Iwata: Scanning ionconductance microscopy for imaging biological samples in liquid: A comparative study with atomic force microscopy and scanning electron microscopy. Micron. 43:1390-1398(2012).[PubMed]
36. Watanabe T, Y Sakai, D Koga, H Bochimoto, Y Hira, M Hosaka, T Ushiki: Aunique ball-shaped Golgi apparatus in the rat pituitary gonadotrope: its functional implications in relation to the arrangement of the microtubule network. J Histochem Cytochem. 60:588-602 (2012).[PubMed]
37. Oyamatsu H, D Koga, M Igarashi,M Shibata, T Ushiki: Morphological assessment of early axonal regeneration in end-to-side nerve coaptation models. J Plast Surg Hand Surg. 46:299-307 (2012) .[PubMed]
38. Moribe H, R Konakawa, D Koga, TUshiki, K Nakamura, E Mekada: Tetraspanin is required for generation of reactive oxygen species by the dual oxidase system in Caenorhabditis elegans. PLoS Genet. 2012 Sep;8(9):e1002957. doi: 10.1371/journal.pgen.1002957. Epub2012 Sep 20. [PubMed]



2011年


39. Hoshi O, T Ushiki: Atomic force microscopy imaging of human metaphase chromosomes in liquid. Methods Mol Biol. 736:109-115 (2011). [PubMed]
40. Iwata F, Y Mizuguchi, H Ko, T Ushiki: Nanomanipulation of biological samples using a compact atomic force microscope under scanning electron microscope observation. J Electron Microsc (Tokyo). 60(6):359-366(2011). [PubMed]
41. Hoshi O, T Ushiki: Replication Banding Patterns in Human Chromosomes Detected Using 5-ethynyl-2'-deoxyuridine Incorporation. Acta Histochem Cytochem. 44(5):233-237 (2011).[PubMed]
42. Yamazaki Y, M Shibata, T Ushiki, K Isokawa, N Sato: Bilateral, asymmetric anomalies of the anterior bellies of digastric muscles. J Oral Sci.53(4):523-527 (2011).[PubMed}



2010年


43. Riva A, B Tandler, T Ushiki, P Usai, R Isola, G Conti, F Loy, CLHoppel: Mitochondria of human Leydig cells as seen by high resolution scanning electron microscopy. Arch Histol Cytol. 73(1):37-44 (2010).
44. Mochizuki E, K Okumura, M Ishikawa, S Yoshimoto, J Yamaguchi, YSeki, K Wada, M Yokohama, T Ushiki, H Tokano, R Ishii, H Shitara, C Taya, K Kitamura, H Yonekawa, Y Kikkawa: Phenotypic and expression analysis of a novel spontaneous myosin VI null mutant mouse. Exp Anim. 59(1):57-71 (2010).
45. Ikeda K, T Mizutani, O Hoshi, T Ushiki, H Haga, K Kawabata: Intermediate structure between chromatin fibers and chromosome revealed bymechanical stretching and SPM measurement. Biochem Biophys Res Commun.400(1):181-186 (2010).[PubMed]
46. Nakayama H, K Takakuda, HN Matsumoto, A Miyata, O Baba, MJTabata, T Ushiki, T Oda, MD McKee, Y Takano: Effects of altered bone remodeling and retention of cement lines on bone quality in osteopetrotic agedc-Src-deficient mice. Calcif Tissue Int. 86:172-83(2010)
47. Iwata, Y Mizuguchi, K Ozawa, T Ushiki: Operation of Self-Sensitive Cantilever in Liquid for Multiprobe Manipulation. Jpn J Appl Phys. 49: 08LB14-1-5 (2010)



2009年


48. Hoshi O, T Ushiki: Structural analysis of human chromosomes by atomic force and light microscopy in relation to the distribution of topoisomerase IIa. Arch Histol Cytol. 72(4-5):245-249 (2009).
49. Iwata F, S Kawanishi, H Aoyama, T Ushiki: Development of a nanomanipulator based on an atomic force microscope coupled with a haptic device: anovel manipulation tool for scanning electron microscopy. Arch Histol Cytol.72(4-5): 271-278 (2009).



2008年


50. Xu XD, H Ueta, S Zhou, C Shi, D Koga, T Ushiki, K Matsuno: Trafficking of recirculating lymphocytes in the rat liver: rapid transmigration into the portal area and then to the hepatic lymph. Liver Int. 28(3):319-330(2008).
51. Picco, L.M., P.G. Dunton, A. Ulcinas, D.J. Engledew, O. Hoshi, T. Ushiki, M.J. Miles: High-speed AFM of human chromosomes in liquid. Nanotechnology 19: 384018 (6pp) (2008)
52. Ushiki, T., M. Shigeno, O. Hoshi: Techniques for imaging human metaphase chromosomes in liquid conditions by atomic force microscopy. Nanotechnology. 19:384022 (2008).
53. Ushiki, T, K Kawabata: Scanning probe microscopy in biological research. In: B Bhushan, H Fuchs, M Tomitori (eds): Applied Scanning Probe Methods X. Biomimetics and industrial applications. Springer, Heidelberg, 2008(pp.285-308)
54. Ushiki, T, O. Hoshi: Atomic force microscopy for imaging human metaphase chromosomes. Chrom Res 16:383-396 (2008)



2007年


55. Kawabata, K., K. Nomura, K. Ikeda, O.Hoshi, D. Fukushi, H. Haga and T. Ushiki: Mechanical approaches to elucidate mechanisms of chromosome condensation at the nano- and micro-level. In: FukuiK, Ushiki T, ed., Chromosome Nanoscience and Technology, CRC Press, pp 1-13,2007
56. Hoshi, O., T. Hirota, E. Kimura, N.Komatsubara and T. Ushiki : Immunocytochemistry for analyzing chromosomes. In:Fukui K, Ushiki T, ed., Chromosome Nanoscience and Technology, CRC Press, pp81-91, 2007
57. Inaga, S., K. Tanaka and T. Ushiki:Transmission and scanning electron microscopy of mammalian metaphasechromosomes. In: Fukui K, Ushiki T, ed., Chromosome Nanoscience and Technology,CRC Press, pp 93-104, 2007
58. Hoshi, O., D. Fukushi and T. Ushiki: Atomicforce microscopy of human chromosomes in relation to their high-orderstructure. In: Fukui K, Ushiki T, ed., Chromosome Nanoscience and Technology,CRC Press, pp 105-117, 2007.
59. Toyabe, S., T. Matsuto, T. Ushiki and K.Akazawa: Image Database and Image Analysis of Chromosome Information. In: FukuiK, Ushiki T, ed., Chromosome Nanoscience and Technology, CRC Press, pp 245-257, 2007.



2006年


60. Hoshi, O. and T. Ushiki: Atomic force microscopy of native human metaphase chromosomes in a liquid. Arch. Histol. Cytol. 69: 73-78 (2006).
61. Koga D. and T.Ushiki: Three-dimensional ultrastructure of the Golgi apparatus in different cells: high-resolution scanning electron microscopy of osumium-macerated tissues. Arch. Histol. Cytol. 69: 357-374 (2006)
62. Oyama T., H. Abe, and T. Ushiki: The connective tissue and glial framework in the optic nerve head of the normal human eyes: light and scanning electron microscopic studies. Arch. Histol. Cytol. 69: 341-356(2006).
63. Walter B.A., V.A. Valera, S. Takahashi, K. Matsuno and T. Ushiki:Evidence of antibody production in the rat cervical lymph nodes after antigenadministration into the cerebrospinal fluid. Arch. Histol. Cytol. 68:37-47(2006).
64. Walter, B.A., V.A. Valera, S. Takahashi, T. Ushiki: The olfactoryroute for cerebrospinal fluid drainage into the peripheral lymphatic system.Neuropathol Appl Neurobiol. 32:388-396(2006)



2005年


65. Fukushi, D., andT. Ushiki: The structure of C-banded human metaphase chromosomes as observed by atomic force microscopy. Arch. Histol. Cytol .68: 81-87 (2005).
66. Toyabe, S., K. Akazawa, D. Fukushi, K. Fukui, and T. Ushiki: CHRONIS: ananimal chromosome image database. Chromosome Res. 13: 593-600 (2005).
67. Niitsuma, J. I., H. Oikawa, E. Kimura, T. Ushiki, and T. Sekiguchi:Cathodoluminescence investigation of organic materials. J. Electron Microsc. 54:325-330 (2005).
68. Watanabe, M, K. Nomura, A. Ohyama, R. Ishikawa,Y. Komiya, K. Hosaka, E. Yamauchi, H. Taniguchi, N. Sasakawa, K. Kumakura,T. Ushiki, O. Sato, M. Ikebe, and M. Igarashi:Myosin-Va regulates exocytosis through the submicromolar Ca2+-dependent bindingof syntaxin-1A. Mol. Biol. Cell 16:4519-4530 (2005).
69. Nomura, K., O. Hoshi, D. Fukushi,T. Ushiki, H. Haga and K. Kawabata:Visualization of elasticity distribution of single human chromosomes by scanningprobe microscopy. Jpn. J. Appl. Phys. 44: 5421-5424 (2005).



2004年


70. Nomura, T., S. Takahashi, and T. Ushiki: Cytoarchitecture of the normal rat olfactory epithelium: light and scanning electron microscopic studies. Arch. Histol. Cytol. 67: 159-170 (2004).
71. Hoshi, O., and T. Ushiki: Neutrophil extravasation in rat mesenteric venules induced by the chemotactic peptide N-formyl-methionyl-luecyl phenylalanine (fMLP), with special attention to a barrier function of the vascular basal lamina for neutrophil migration. Arch. Histol. Cytol. 67: 107-114 (2004).
72. Hoshi, O., R. Owen, M. Miles, andT. Ushiki: Imaging of human metaphasechromosomes by atomic force microscopy in liquid. Cytogenet. Genome Res. 107:28-31 (2004).
73. Kimura, E., T. Sekiguchi, H. Oikawa, J.Niitsuma, Y. Nakayama, H. Suzuki, M. Kimura, K. Fujii, andT. Ushiki: Cathodoluminescence imaging for identifying uptaken fluorescence materials in Kupffer cells using scanning electron microscopy. Arch. Histol. Cytol. 67: 263-270 (2004).
74. Kimura, E., O. Hoshi, andT. Ushiki: Atomic force microscopy ofhuman metaphase chromosomes after differential staining of sister chromatids.Arch. Histol. Cytol.67: 171-177 (2004).
75. Nemoto, M, Y. Morita, Y. Mishima, S. Takahashi,T. Nomura, T. Ushiki, T. Shiroishi, Y. Kikkawa, H. Yonekawa, and R. Kominami: Ahl3,a third locus on mouse chromosome 17 affecting age-related hearing loss. Biochem.Biophys. Res. Commun. 324:1283-1288 (2004).



2003年


76. Murata, H., M. Yagi, M. Kubota, and T. Ushiki: Embryonic development of the myenteric nerve plexus in the rat small intestine as revealed by light and scanning electron microscopy. Arch. Histol. Cytol. 66: 453-468 (2003).
77. Mizuta, R., K. Iwai, M. Shigeno, M. Mizuta, T. Uemura, T. Ushiki, and D. Kitamura: Molecular visualization of immunoglobulin switch region RNA/DNA complex by atomic force microscope. J. Biol. Chem. 278: 4431-4434 (2003).
78. Ushiki, T.: Atomic force microscopy for imaging living organisms: from DNA to cell motion. In: (ed.by H. Fujita) Micromachines as tools for nanotechnology. Springer,Berlin, 2003 (p.121-130)



2002年


79. Shinohara, H., M. Fukushi, M. Higuchi, M. Oie, O. Hoshi,T. Ushiki,J. Hayashi, and M. Fujii: Chromosome binding site of late ncy-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus is essential for persistentepisome maintenance and is functionally replaced by histone H1. J. Virol. 76:12917-12924 (2002).
80. Takahashi, S., J. Hitomi, Y. Satoh, T.Takahashi, H. Asakura, andT. Ushiki:Fine structure of the mouse portal vein in relation to its peristalticmovement. Arch Histol Cytol65: 71-82 (2002).
81. Yamada, T., E. Suzuki, F. Gejyo, andT. Ushiki: Developmental changes in thestructure of the rat fetal lung, with special reference to the airway smoothmuscle and vasculature. Arch. Histol. Cytol. 65: 55-69 (2002).
82. Yoshino, T., S. Sugiyama, S. Hagiwara,T. Ushiki, and T. Ohtani: Simultaneouscollection of topographic and fluorescent images of barley chromosomes byscanning near-field optical/atomic force microscopy. J. Electron Microsc. 51:199-203 (2002).
83. Yamamoto, S., J.Hitomi, S. Sawaguchi, H. Abe, M. Shigeno, andT. Ushiki: Observation of human corneal and scleral collagenfibrils by atomic force microscopy. Jpn. J. Ophthalmol.46: 496-501 (2002).
84. Kimura, E., J. Hitomi, andT. Ushiki: Scanning near fieldoptical/atomic force microscopy of bromodeoxyuridine-incorporated humanchromosomes. Arch.Histol. Cytol. 65: 435-444 (2002).
85. Sone, T., M. Iwano, S. Kobayashi, T.Ishihara, N. Hori, H. Takata,T. Ushiki,S. Uchiyama, and K. Fukui: Changes in chromosomal surface structure bydifferent isolation conditions. Arch. Histol. Cytol. 65: 445-455 (2002).
86. Iwabuchii, S., T. Mori, K. Ogawa, K. Sato,M. Saito, Y. Morita,T. Ushiki, andE. Tamiya: Atomic force microscope-based dissection of human metaphasechromosomes and high resolutional imaging by carbon nanotube tip. Arch. Histol. Cytol. 65: 473-479 (2002).
87. Hashizume, H., andT. Ushiki: Three-dimensional cytoarchitecture of angiogenic bloodvessels in a gelatin sheet implanted in the rat skeletal muscular layers. Arch.Histol. Cytol. 65: 347-357 (2002).
88. Ohtani, T., M. Shichirii, D. Fukushi, S.Sugiyama, T. Yoshino, T. Kobori, S. Hagiwara, andT. Ushiki: Imaging of chromosomes at nano-meter scale resolutionusing scanning near-field optical/atomic force microscopy. Arch. Histol. Cytol.65: 425-434 (2002).
89. Naito, T., T. Kawamura, M. Bannai, T. Kosaka,H. Kameyama, K. Shimamura, O. Hoshi,T. Ushiki,K. Hatakeyama, and T. Abo: Simultaneous activation of natural killer T cellsand autoantibody production in mice injected with denatured syngeneic livertissue. Clin. Exp. Immunol. 129:397-404 (2002).
90. Shinohara, H., M. Fukushi, M. Higuchi, M. Oie, O. Hoshi, T. Ushiki, J. Hayashi, M. Fujii: Chromosome binding site of latency-associated nuclear antigen of Kaposi'ssarcoma-associated herpesvirus is essential for persistent episome maintenanceand is functionally replaced by histone H1. J Virol. 76:12917-12924 (2002).
91. Ushiki, T.: Collagenfibers, reticular fibers and elastic fibers. A comprehensive understanding from a morphological viewpoint. Arch Histol Cytol65: 109-26 (2002).
92. Ushiki, T., O. Hoshi, K. Iwai, E. Kimura, and M. Shigeno: The structureof human metaphase chromosomes: its histological perspective and new horizons by atomic force microscopy. Arch Histol Cytol65: 377-90 (2002).



2001年


93. Higuchi, K., K. Kumaki, Y. Aizawa, and T. Ushiki: A KOH-collagenase digestion method for scanning electron microscopic studies of vascular smooth muscle fibers in the human heart. Ital. J. Anat. Embryol. 106: 197-202 (2001)
94. Sunami, E., H. Kanazawa, H. Hashizume, M. Takeda, K. Hatakeyama, and T. Ushiki: Morphological characteristics of Schwann cells in the islets of Langerhans of the murine pancreas. Arch. Histol. Cytol. 64: 191-201 (2001).
95. Iino,N., F. Gejyo, M. Arakawa, and T. Ushiki: Three-dimensional analysis of nephrogenesis in the neonatal rat kidney: lightand scanning electron microscopic studies. Arch. Histol. Cytol. 64: 179-190(2001).
96. Sonoda, E., T. Matsusaka, C. Morrison, P. Vagnarelli, O. Hoshi, T. Ushiki, K. Nojima, T. Fukagawa, I. C. Waizenegger, J. M. Peters, W. C. Earnshaw, and S. Takeda: Scc1/Rad21/Mcd1 is required for sister chromatid cohesion and kinetochore function in vertebrate cells. Dev. Cell 1: 759-770(2001).
97. Hoshi, O., and T. Ushiki: Three-dimensional structure of G-banded human metaphase chromosomes observed by atomic force microscopy. Arch. Histol. Cytol. 64: 475-482 (2001).
98. Yamada,T., and T. Ushiki: Cytoarchitecture of the bronchiolar wall in the rat lung: scanning electron microscopic studies. Ital. J. Anat. Embryol. 106: 387-393 (2001).
99. Iino,N., F. Gejyo, M. Arakawa, and T. Ushiki: The three-dimensional structure of the neonatal mouse kidney as revealed by scanning electron microscopy after KOH treatment. Ital. J. Anat. Embryol. 106:415-421 (2001).
100. Tagawa,M., K. Higuchi, M. Chinushi, T. Washizuka, T. Ushiki, N. Ishihara, and Y. Aizawa: Myocardium extending from the left atrium onto the pulmonary veins: A comparison between subjects with and without atrial fibrillation. Pacing Clin. Electrophysiol. 24: 1459-1463(2001).
101. Wada,T., Y. Wakabayashi, S. Takahashi, T. Ushiki,Y. Kikkawa, H. Yonekawa, R. Kominami: A point mutation in a cadherin gene,Cdh23, causes deafness in a novel mutant, Waltzer mouse niigata. Biochem. Biophys. Res. Commun. 283,113-117 (2001).
102. Ushiki, T.: Atomic force microscopy and its related techniques in biomedicine. Ital J Anat Embryol 106: 3-8 (2001).



2000年


103. Yamamoto, S., H. Hashizume, J. Hitomi, M. Shigeno, S. Sawaguchi, H. Abe,and T. Ushiki: The subfibrillar arrangement of corneal and scleral collagen fibrils as revealed by scanning electron and atomic force microscopy. Arch. Histol.Cytol. 63: 127-135 (2000).
104. Higuchi,K., H. Hashizume, Y. Aizawa, and T.Ushiki: Scanning electron microscopic studies of the vascular smooth musclecells and pericytes in the rat heart. Arch. Histol. Cytol. 63: 115-126 (2000).
105. Haga,H., S. Sasaki, K. Kawabata, E. Ito, T. Ushiki, and T. Sambongi: Elasticity mapping of living fibroblasts by AFMand immunofluorescence observation of the cytoskeleton. Ultramicroscopy 82:253-258 (2000).
106. Tango,M., E. Suzuki, F. Gejyo, and T. Ushiki:The presence of specialized epithelial cells on the bronchus-associated lymphoid tissue (BALT) in the mouse. Arch. Histol. Cytol. 63: 81-89 (2000).
107. Haga,H., M. Nagayama, K. Kawabata, E. Ito, T. Ushiki, and T. Sambongi: Time-lapse viscoelastic imaging of living fibroblasts using force modulation mode in AFM. J. Electron Microsc. 49: 473-481(2000).
108. Ohtani,T., T. Yoshino, T. Ushiki, S. Hagiwara, and T. Maekawa: Structure of rice starch granules in nanometre scaleas revealed by atomic force microscopy. J. Electron Microsc. 49: 487-489(2000).
109. Tojima,T., Y. Yamane, H. Takagi, T. Takeshita, T. Sugiyama, H. Haga, K. Kawabata, T. Ushiki, K. Abe, T. Yoshioka, and E. Ito: Three-dimensional characterization of interior structures of exocytotic apertures of nerve cells using atomic force microscopy. Neuroscience 101: 471-481(2000).
110. Yamamoto,S., F. Nakamura, J. Hitomi, M. Shigeno, S. Sawaguchi, H. Abe, and T. Ushiki: Atomic force microscopy of intact and digested collagen molecules. J. Electron Microsc. 49: 423-427 (2000).
111. Ohtani,T., T. Yoshino, T. Ushiki, S. Hagiwara and T. Maekawa: Structure of rice starch granules in nanometre scaleas revealed by atomic force microscopy. J. Electron Microsc. 49: 487-489 (2000).
112. Ushiki, T, S. Yamamoto, J. Hitomi, S. Ogura, T. Umemoto and M. Shigeno: Atomic force microscopy of living cells. Jpn J Appl Phys 39:3761-3764 (2000)



1999年


113. Ushiki, T., J. Hitomi, T. Umemoto, S. Yamamoto, H. Kanazawa, and M. Shigeno: Imaging of living cultured cells of an epithelial nature by atomic force microscopy. Arch Histol Cytol 62: 47-55 (1999).
114. Hashimoto,Y.,T. Ushiki, T. Uchida, J. Yamada and T. Iwanaga: Scanning electron microscopic observation of apical sites of open-type paraneurons in the stomach, intestine and urethra. Arch. Histol. Cytol., 62:181-189 (1999).
115. Hoshi, O., and T. Ushiki: Scanning electron microscopic studies on the route of neutrophil extravasation in the mouse after exposure to the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP). Arch. Histol. Cytol. 62: 253-260 (1999).
116. Hashizume, H., J. Hitomi, and T. Ushiki: Growth of collagen fibrils produced by human osteosarcoma cells: high-resolution scanning electron microscopy. Arch. Histol. Cytol. 62: 327-335 (1999)



1998年


117. Hashizume, H., S. Itoh, K. Tanaka, and T. Ushiki: Direct observation oft-butyl alcohol frozen and sublimated samples using low-vacuum scanningelectron microscopy. Arch. Histol. Cytol. 61: 93-98 (1998).
118. Yoshida,K., M. Yoshimoto, K. Sasaki, T. Ohnishi, T. Ushiki, J. Hitomi, S. Yamamoto, and M. Sigeno: Fabrication of a new substrate for atomic force microscopic observation of DNA molecules from an ultrasmooth sapphire plate. Biophys. J. 74: 1654-1657 (1998).
119. Sasaki,S., M. Morimoto, H. Haga, K. Kawabata, E. Ito,T. Ushiki, K. Abe, and T. Sambongi: Elastic properties of living fibroblasts as imaged using force modulation mode in atomic force microscopy. Arch. Histol. Cytol. 61: 57-63 (1998).
120. Narita,J., C. Miyaji, H. Watanabe, S. Honda, T. Koya, H. Umezu,T. Ushiki, S. Sugahara, T. Kawamura, M. Arakawa and T. Abo: Differentiation of forbidden T cell clones and granulocytes in the parenchymal space of the liver in mice treated with estrogen. Cell. Immunol. 185: 1-13 (1998).
121. Yamane,Y., D. Hatakeyama, T. Tojima, K. Kawabata,T.Ushiki,S. Ogura, K. Abe and E. Ito: Fine surface images that reflect cytoskeletal structures in cultured glial cells by atomic force microscopy. Jpn. J. Appl. Phys. 37: 3849-3854 (1998).
122. Tojima,T., D. Hatakeyama, Y. Yamane, K. Kawabata,T. Ushiki, S. Ogura, K. Abe and E. Ito: Comparative atomic force and scanning electron microscopy for fine structural images of nerve cells. Jpn. J. Appl. Phys. 37:3855-3859 (1998).
123. Ushiki, T., and K. Abe: Identification of arterial and venous segments of blood vessels using alkaline phosphatase staining of ink/gelatin injected tissues. Arch. Histol. Cytol. 61: 215-219(1998).
124. Tanaka,K., S. Inaga, A. Iino,T. Ushiki and S. Saito: Fine structure of hydrous chromosomes observed by low vacuum scanning electron microscopy. Arch. Histol. Cytol. 61: 337-342 (1998).
125. Hashizume,H., M. Tango, and T. Ushiki: Three-dimensional cytoarchitecture of rat pulmonary venous walls: a light and scanning electron microscopic study. Anat. Embryol. 198: 473-480 (1998).
126. Ushiki, T., H. Hashizume, S. Itoh, K. Kuboki, S. Saito, and K. Tanaka: Low-voltage backscattered electron imaging of non-coated biological samples in a low-vacuum environment using a variable-pressure scanning electron microscope with a YAG-detector. J. Electron Microsc. 47: 351-354 (1998).
127. Nagano, T., Y. Iwakura, T. Ushiki, and H. Nawa: Development of neurites, dendritic filopodiaand synaptic spines in hippocampal low-density culture. In:(ed. by K. Uemura,K. kawamura and T. Tazaki) Neural Development. Springer, 1998 (p. 378-393)
128. Abe, K., H. Hashizume, T. Ushiki, J. Kinoshita, and R. Shibata: Shape of osteoblasts and osteocytes tell their function and differentiation. In: (ed. byS. Yagihashi, T. Kachi and M. Wakui) Dynamic cells: Cell biology of the 21th century. Elsevier, Amsterdam,1998 (p. 113-123).



1997年


129. Ushiki, T., and M. Takeda: Three-dimensionalultrastructure of the perivascular space in the rat thymus. Arch. Histol. Cytol.60: 89-99 (1997).
130. Yamamoto,S., J. Hitomi, M. Shigeno, S. Sawaguchi, H. Abe, and T. Ushiki: Atomic force microscopic studies of isolated collagen fibrils of the bovine cornea and sclera. Arch. Histol. Cytol. 60: 371-378(1997).
131. Hashizume,H., K. Abe, and T. Ushiki: Detection of mineral density on the surface of mouse parietal bones: backscattered electron imaging of low accelerating voltage scanning electron microscopy. Arch.Histol. Cytol. 60: 195-204 (1997).
132. Murakumo, M.,K. Nonomura, T. Yamashita, and T. Ushiki: Structural changes of collagen components and diminution of nerves in congenital ureteropelvic junction obstruction. J. Urol. 157:1963-1968 (1997).
133. Nagayama, S., T. Tojima, M. Morimoto,S. Sasaki, K. Kawabata,T . Ushiki, K Abe and E. Ito: Practical scan speed in atomic force microscopy for live neurons in a physiological solution. Jpn. J. Appl. 36: 3877-3880 (1997).
134. Ushiki,T., and S. Watanabe: Distribution and ulrastructure of the autonomic nervesin the mouse pancreas: Microsc. Res. Technique. 37: 399-406 (1997).
135. Ushiki, T., S. Ogura, J. Hitomi, T. Umemoto and M. Shigeno: Observations of cells and tissues by atomic force microscopy. In:(ed. by P. M. Motta) Recent advances in microscopy of cells, tissues and organs.Antonio Delfino Editore, Roma, 1997 (p.59-63).



1996年


136. Hayashi, M., S. Fujimoto, H. Takano, T. Ushiki, K.Abe, H. Ishikura, M. C. Yoshida, C. Kirchhoff, T. Ishibashi and M. Kasahara: Characterization of a human glycoprotein with a potential role in sperm-eggfusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1). Genomics 32: 367-374 (1996).
137. Amemiya, F., T. Ushiki, R. C. Goris, Y. Atobe and T. Kusunoki: Ultrastructure of the crotaline snake infrared pit receptors: SEM confirmation of TEM findings. Anat. Rec. 246: 135-146 (1996).
138. Nagayama, S., M. Morimoto, K. Kawabata, Y. Fujito, S. Ogura, K. Abe,T. Ushikiand E. Ito: AFM observation of three-dimensional fine structural changes in living neurons.Bioimages 4: 111-116 (1996).
139. Muramatsu, H., N. Chiba,K. Nakajima, T. Ataka, M. Fujihira, J. Hitomi, and T. Ushiki: Fluorescence imaging and spectroscopy of biomaterials inair and liquid by scanning near-field optical/atomic force microscopy. Scanning.Microsc. 10: 975-982 (1996).
140. Ushiki, T., J. Hitomi,S. Ogura, T. Umemoto, and M. Shigeno: Atomic force microscopy in histology and cytology. Arch Histol Cytol59: 421-31 (1996).



1995年


141. Ushiki, T., O. Ohtani and K. Abe: Scanning electron microscopic studies of reticular framework in the rat mesenteric lymph node.Anat. Rec. 241: 113-122 (1995).
142. Murakumo,M.,T. Ushiki, K. Abe, K. Matumura, Y. Shinno and T. Koyanagi: Three-dimensional arangement of collagen and elastin fibers in the human urinary bladder: a scanning electron microscopic study. J. Urol. 154:251-256 (1995).
143. Hashizume, H.,T. Ushikiand K. Abe: A histologigal study of the cardiac muscle of the human superior and inferior venae cavae. Arch. Histol. Cytol. 58: 457-464 (1995).
144. Mikuni, H., S. Fukuda, Y. Inuyama, T. Ushiki, K. Abe: The three-dimensional fibrillar arrangement ofthe basilar membrane in the mouse cochlea. Eur. Arch. Otorhinolaryngol. 252:495-498 (1995).
145. Ushiki, T.: Connective tissue sheaths of peripheral nerves. In: (ed. by M. Kida and M. Horiguchi) An active direction to the future of peripheral nerve research. Kyobunsha,Sapporo, 1995 (p. 31-40).